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TIME-LINE FOR NSF PROJECT 0115103


A Sequence-Indexed Library of Insertion Mutations in the Arabidopsis Genome
Joseph R. Ecker, Principal Investigator
The Salk Institute for Biological Studies



YEAR 1 (September 1, 2001 - August 31, 2002)

September 1, 2001
Initiation of funding

October 1, 2001
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

November 1, 2001
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

December 1, 2001
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

January 1, 2002
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

February 1, 2002
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

March 1, 2002
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

April 1, 2002
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

May 1, 2002
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

June 1, 2002
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

July 1, 2002
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

August 1, 2002
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

YEAR 2 (September 1, 2002 - August 31, 2003)

September 1, 2002
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

October 1, 2002
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

November 1, 2002
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

December 1, 2002
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

January 1, 2003
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

February 1, 2003
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC

Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

March 1, 2003
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

April 1, 2003
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR

May 1, 2003
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR. These sequences will be produced from lines that failed the first time around.

June 1, 2003
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR
Begin direct screening of DNA pools for genes not found by sequencing

July 1, 2003
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR
Continue direct screening of DNA pools for genes not found by sequencing

August 1, 2003
Send 0.1 - 0.2 ml (2,500 to 5,000) seeds of all sequenced T-DNA gene insertion mutants to the ABRC
Generate ~6,250 T-DNA flanking sequences and submit high quality reads to GenBank, and TAIR
Continue directed screening of DNA pools for insertions in remaining genes.

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